Published 2011 | Version v1
Dataset Open

HeLa CHMP4A+VPS4DN

  • 1. ROR icon California Institute of Technology

Contributors

Description

HeLa cells transfected with CHMP4A & VPS4DN constructs

Technical info

Tilt Series Date: 2011-02-09

Data Taken By: Cora Woodward

Species / Specimen: HeLa cells

Strain: ATCC

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with polyfect

Sample Preparation: manual blotting

Attached Files

11feb09b_C50-4d_020.st, Tilt Series (Pixel Size 0.5 nm), 981.5 MB

11feb09b_C50-4d_020_full.rec, Reconstruction (Pixel Size 2.0 nm), 478.2 MB

Files

keyimg_cwl2011-02-09-10.jpg

Files (1.5 GB)

Name Size
981.5 MB (external) Download
478.2 MB (external) Download
md5:b6fd9b18d7ae3af1eefbfd0872584730
1.1 MB Preview Download
md5:54f93a471bb33b5473c0be77d157ab4d
7.3 MB Preview Download

Additional details

Identifiers

DOI
10.22002/z0j3z-nwg09
TILT_SERIES_ID
cwl2011-02-09-10

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2011-02-09
Created
2015-09-16
Updated
2015-09-16