HeLa siRNA tfx CHMP2A/B infx pNLGFP
Description
Technical info
Tilt Series Date: 2011-05-31
Data Taken By: Cora Woodward
Species / Specimen: HeLa cells
Strain: ATCC
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod
Collaborators and Roles: CHMP2A and 2B siRNAs provided by Wes Sundquist. pNLGFP proviral construct provided by Irvin Chen UCLA. Virus prepared at Caltech by Cora Woodward. Cells transfected with siRNA and then infected, frozen and imaged at Caltech by Cora Woodward
Purification / Growth Conditions / Treatment: whole cells grown on grids and infected and siRNA transfected
Sample Preparation: manual blotting
Attached Files
11may31_4_orig_2_full.rec, Reconstruction (Pixel Size 2.0 nm), 681.3 MB
11may31_4_orig_2.st, Tilt Series (Pixel Size 0.5 nm), 924.9 MB
11may31_4_orig_2.tiff, file associated to 3D Image #68489, 452.3 kB
Files
11may31_4_orig_2.tiff
- This file is an external reference and not stored directly in this repository. To access its content, please download it and open it locally.
Additional details
Identifiers
- DOI
- 10.22002/z2gwm-zey86
- TILT_SERIES_ID
- cwl2011-05-31-2
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2011-05-31
- Created
-
2015-09-16
- Updated
-
2015-09-16