B. diazoefficiens, hopanoids
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2019-11-06
Data Taken By: Yuxi Liu
Species / Specimen: Bradyrhizobium diazoefficiens
Strain:
Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 33000x.
Microscope: Caltech Titan Krios
Acquisition Software: Serial EM
Upload Method: pipeline
Processing Software Used: None
Collaborators and Roles: Brittany Belin (Newman Lab, CalTech): strain construction, culture growth
Purification / Growth Conditions / Treatment: log phase, treated with naringenin
Sample Preparation: Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid
Attached Files
WT_LG_64step2_10.mrc, Tilt Series (Pixel Size 2.678 nm), 3.1 GB
Files
keyimg_ylx2019-11-06-7.jpg
Additional details
Identifiers
- DOI
- 10.22002/z3ekp-n2e58
- TILT_SERIES_ID
- ylx2019-11-06-7
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2019-11-06
- Created
-
2019-11-24
- Updated
-
2020-01-02