Published 2015
| Version v1
Dataset
Open
PW3654
Description
Pseudomonas aeruginosa, strain PW3654.Acquired from the Transposon mutant collection. PW3654- lacZwp06q3 (CheW of F6 system)
Technical info
Tilt Series Date: 2015-02-23
Data Taken By: Ariane Briegel
Species / Specimen: Pseudomonas aeruginosa
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: 10.0 μm, magnification: 34000x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Purification / Growth Conditions / Treatment: Streak on LB plates and incubate overnight at 37°C- Inoculate 5 mL MOPS Minimal Media Limited Nitrogen from plate and grow at 30°C for ~24 hours
Attached Files
PseudomonasdeltaFF6CheWNEWFEB0009.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB
PseudomonasdeltaFF6CheWNEWFEB0009_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB
Files
keyimg_aba2015-02-23-51.jpg
Additional details
Identifiers
- DOI
- 10.22002/z7jxk-bet67
- TILT_SERIES_ID
- aba2015-02-23-51
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-02-23
- Created
-
2015-02-24
- Updated
-
2015-02-25