Published 2012 | Version v1
Dataset Open

E. coli (FB76)

Creators

  • 1. ROR icon California Institute of Technology

Description

Contains MreB-mCherry sandwich fusion as sole source of MreB from WT promoter.

Technical info

Tilt Series Date: 2012-05-07

Data Taken By: Matt Swulius

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: None μm, magnification: 23000x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFTomo

Collaborators and Roles: Gift from Piet de Boer

Purification / Growth Conditions / Treatment: LB, 30 Degrees Celsius, OD600 ~ 0.6

Attached Files

20120507_FB76_M2.mrc, Tilt Series (Pixel Size 0.95 nm), 1.1 GB

20120507_FB76_M2_part130_68.rec, Reconstruction (Pixel Size 1.9 nm), 825.8 MB

Files

keyimg_mst2012-05-07-4.jpg

Files (1.9 GB)

Name Size
1.1 GB (external) Download
825.8 MB (external) Download
md5:e3cd9ff104270b658d4fbc7174ccb7cf
1.2 MB Preview Download
md5:5bd41a4a03476eedebe7259108dcd5b7
9.8 MB Preview Download

Additional details

Identifiers

DOI
10.22002/zb2b7-ram69
TILT_SERIES_ID
mst2012-05-07-4

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2012-05-07
Created
2012-05-07
Updated
2012-07-02