MEFs (Rho-) Chan Lab
Description
Technical info
Tilt Series Date: 2012-05-29
Data Taken By: Cora Woodward
Species / Specimen: Murine embryonic fibroblast (MEFs)
Strain: Primary cells
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod
Collaborators and Roles: Mefs from Chan Lab. Contact person in Chan lab was H. Ngo. Cells were grown on grids, frozen and imaged at Caltech by Cora Woodward
Purification / Growth Conditions / Treatment: whole cells grown on grids
Sample Preparation: manual blotting
Attached Files
12may29_C135_1_MEFnomitoDNA_AT2BT20013_full.rec, Reconstruction (Pixel Size 2.0 nm), 2.9 GB
12may29_C135_1_MEFnomitoDNA_AT2BT20013.mrc, Tilt Series (Pixel Size 0.5 nm), 677.3 MB
Files
keyimg_cwl2012-05-29-22.jpg
Additional details
Identifiers
- DOI
- 10.22002/zb8gh-whq02
- TILT_SERIES_ID
- cwl2012-05-29-22
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-05-29
- Created
-
2015-09-16
- Updated
-
2015-09-16