CheA-GE8-L2

Description

Technical info

Tilt Series Date: 2012-10-29

Data Taken By: Ariane Briegel

Species / Specimen: Escherichia coli

Strain: RP 437

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: °, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 0x.

Acquisition Software: FEI Tomo

Upload Method: webload

Processing Software Used: IMOD

Collaborators and Roles: Carly Carter, Dan Feldheim

Purification / Growth Conditions / Treatment: cells grown to log phase in TB, induced with 14 mM IPTG for 3 hours, lysed with penicillin for 1 hour, add DNAse buffer, DNAse for 20 min, add AgNO3 (0.014g AgNO3 in 1ml HEPES pH 7.2) and 50µl to 1 ml, incubated 20 min, spin down at 6krpm

Sample Preparation: plunge freeze on Quantifoil R2/2 in liquid ethane/propane mix

Attached Files

CheA_AG-1_24.st, Tilt Series (Pixel Size 0.0 nm), 1.1 GB

CheA_AG-1_24.rec, Reconstruction (Pixel Size 0.0 nm), 2.7 GB