Caulobacter crescentus
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2006-02-08
Data Taken By: Prabha Dias
Species / Specimen: Caulobacter crescentus
Strain: NA1000
Tilt Series Settings: Single Axis, tilt range: (-61.0°, 31.0°), step: 0.7°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -12.0 μm, magnification: 0x.
Acquisition Software: UCSF Tomo
Upload Method: rundir
Processing Software Used: imod, bsoft
Collaborators and Roles: specimen prep, data collection & processing: D. Prabha Dias
Purification / Growth Conditions / Treatment: standard PYE media (Shapiro, Brun, etc. recipe); cultured in 50ml flask; exponential phase; glutaraldehyde-fixed on grid; see DPD notebook Feb 2-3, 2006
Sample Preparation: box 177, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1 sec, drain 1.5 sec; see DPD notebook Aug 14, 2006
Attached Files
06feb08t01.mrc, Tilt Series (Pixel Size 1.2 nm), 1.5 GB
06Feb08t01trbn_full.rec, Reconstruction (Pixel Size 2.4 nm), 1.5 GB
Files
keyimg_pdd2006-02-08-1.jpg
Additional details
Identifiers
- DOI
- 10.22002/zckm5-b9q55
- TILT_SERIES_ID
- pdd2006-02-08-1
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2006-02-08
- Created
-
2008-06-18
- Updated
-
2009-07-14