Staphylococcus aureus_WT

Description

Technical info

Tilt Series Date: 2017-07-20

Data Taken By: Debnath Ghosal

Species / Specimen: Staphylococcus aureus

Strain: subsp. aureus str. Newman

Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 240.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Tracy Palmer, University of Dundee. Data collected by Debnath Ghosal.

Purification / Growth Conditions / Treatment: Cells were grown in TSA and in TSB (Oxoid TSA and TSB from Thermo Fisher 10525943 & 10137562). Dilute an overnight culture 1/100 and grow for ~4 hr (to OD 2) then freeze.

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 6 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.

Attached Files

Saureus_WT_201707200014.mrc, Tilt Series (Pixel Size 0.39 nm), 1.5 GB

Saureus_WT_201707200014.rec, Reconstruction (Pixel Size 1.56 nm), 889.0 MB