Myxococcus xanthus delta-pilM
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-12-17
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.
Microscope: Caltech Polara
Acquisition Software: Undefined
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. 20 nm gold beads.
Attached Files
20131217_YWC058_10015.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB
20131217_YWC058_10015.rec, Reconstruction (Pixel Size 1.28 nm), 1.4 GB
Files
keyimg_ycw2013-12-17-15.jpg
Additional details
Identifiers
- DOI
- 10.22002/zqdd1-4ps78
- TILT_SERIES_ID
- ycw2013-12-17-15
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-12-17
- Created
-
2013-12-17
- Updated
-
2013-12-19