Published 2015 | Version v1
Dataset Open

pED208_pilated

  • 1. ROR icon California Institute of Technology

Description

E coli strain with plasmid pED208 overexpresses T4SS and F-like pili

Technical info

Tilt Series Date: 2015-01-14

Data Taken By: Elitza Tocheva

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Cells were obtained from Laura Frost

Purification / Growth Conditions / Treatment: o/n liquid LB

Sample Preparation: Vitrobot

Attached Files

pED208b_6um0021.mrc, Tilt Series (Pixel Size 0.5 nm), 3.4 GB

pED208b_6um0021_full.rec, Reconstruction (Pixel Size 2.0 nm), 445.4 MB

Files

keyimg_etl2015-01-14-121.jpg

Files (3.9 GB)

Name Size
md5:2e1bbd847c4b118b8bad9cca0c0f4bf5
1.1 MB Preview Download
md5:c4a872e7af49c606f2326fc290fa5aff
13.9 MB Preview Download
3.4 GB (external) Download
445.4 MB (external) Download

Additional details

Identifiers

DOI
10.22002/zqxt0-gkp82
TILT_SERIES_ID
etl2015-01-14-121

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-01-14
Created
2015-01-16
Updated
2015-01-16