Oil well Actinobacterium
Description
Technical info
Tilt Series Date: 2016-04-12
Data Taken By: Yi-Wei Chang
Species / Specimen: Unspecified
Strain: None
Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 2.0°, constant angular increment, dosage: 125.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Fixed cells received from Meng Wang/ Xiao-Lei Wu Lab at Peking University, China
Purification / Growth Conditions / Treatment: Firstly wash cells using PBS for 3 times, then add proper volum of 2.5% glutaraldehyde followed 1 hour room temp incubation (done in Peking), then send to Caltech for freezing.
Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
Attached Files
20160410_YWC210_20007.mrc, Tilt Series (Pixel Size 0.48 nm), 1.5 GB
20160410_YWC210_20007_full.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB
Files
keyimg_ycw2016-04-12-6.jpg
Additional details
Identifiers
- DOI
- 10.22002/zrgtx-8rj68
- TILT_SERIES_ID
- ycw2016-04-12-6
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-04-12
- Created
-
2016-04-12
- Updated
-
2016-04-26