Caltech Tomography Archive
Published 2019 | Version v1
Dataset Open

C. crescentus NA1000 Tad pilus

Wang, Steven1
  • 1. ROR icon California Institute of Technology

Description

C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.

Files

keyimg_swy2019-08-17-136.jpg
Files (28.3 MB)
Name Size
keyimg_swy2019-08-17-136.jpg
md5:dd52766808c778ac3641b41f31a257c0
28.3 MB Preview Download

Technical info

Tilt Series Date: 2019-08-17

Data Taken By: Steven Wang

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -6.0 μm, magnification: 26000x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: None

Collaborators and Roles: Patrick Viollier and Lujia Zhang

Purification / Growth Conditions / Treatment: Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.

Sample Preparation: 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-136/rawdata/

caulobacter_na1000_wt_137_af.mrc, Tilt Series (Pixel Size 0.336 nm), 3.9 GB Download

caulobacter_na1000_wt_137_af.mrc.mdoc, 812.0 B Download

Additional details

Identifiers Funding Dates
Created:
June 4, 2023
Modified:
August 26, 2025