Bdellovibrio attack-phase

Description

Technical info

Tilt Series Date: 2013-08-20

Data Taken By: Yi-Wei Chang

Species / Specimen: Bdellovibrio bacteriovorus

Strain: HD100

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain from Liz Sockett, Cells prepared by Lee Rettberg

Purification / Growth Conditions / Treatment: Cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, mixed with purified WM3433 mini E. coli for 10 min before freezing

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid

ycw2013-08-20-18_slicer10315.jpg: nucleoid, Bdellovibrio bacteriovorus ycw2013-08-20-18_slicer14179.jpg: flagellar motor; flagellum; Bdellovibrio bacteriovorus

Attached Files

20130820_Bdellovibrio+WM3433_10008.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB

20130820_Bdellovibrio+WM3433_10008_part131_5.rec, Reconstruction (Pixel Size 1.56 nm), 534.5 MB