H. pylori 26695 WT / AGS
Description
Technical info
Tilt Series Date: 2016-11-02
Data Taken By: Yi-Wei Chang
Species / Specimen: Helicobacter pylori
Strain: 26695
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain and sample prepared by Carrie Shaffer
Sample Preparation: Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ml gold+ 25 ml 10% BSA x4, resuspend in 30 ul). Blot for 5 sec (1 sec longer than usual to remove more solution). Ethane/propane mix.
Attached Files
20161102_YWC229_20018.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
20161102_YWC229_20018_part121_11.rec, Reconstruction (Pixel Size 1.92 nm), 534.5 MB
Files
keyimg_ycw2016-11-02-20.jpg
Additional details
Identifiers
- DOI
- 10.22002/zvnw6-err55
- TILT_SERIES_ID
- ycw2016-11-02-20
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-11-02
- Created
-
2016-11-03
- Updated
-
2016-11-03