Movie 1 Climbing behavior on smooth surface in wild-type male flies Wild-type female flies (CS strain) are able to climb the smooth wall of the culture bottle.

Movie 6 Climbing behavior on rough surface in nwB /CyO female mutants nwB /CyO female flies are able to climb the rough wall of the filter paper.

Movie 5 Climbing behavior on rough surface in wild-type female flies Wild-type female flies of the CS strain are able to climb the rough wall of the filter paper.

Movie 3 Climbing behavior on smooth surface in nwB /CyO male mutants About half of the nwB /CyO male mutants are unable to climb the smooth wall of the culture bottle.

Movie 4 Climbing behavior on smooth surface in nwB /CyO female mutants More than half of the nwB /CyO female mutants are unable to climb the smooth wall of the culture bottle.

Movie 2 Climbing behavior on smooth surface in wild-type female flies Wild-type male flies (CS strain) are able to climb the smooth wall of the culture bottle.

Movie 2 Blister wings caused by precocious cell death in a hidW mutant Although a hidW /TM6b mutant fly showed normal wing extension behavior, adhesion of two layers of the dorsal and ventral cuticles of the wings was disrupted by precocious cell death, resulting in the formation of blister wings during extension.

Movie 1 Wing spreading after eclosion in a wild-type fly A newly eclosed wild-type fly (CS strain) with folded wings walked around, and then expanded it wings within 30 min after eclosion.