This protocol details the step-by-step procedure to amputate adult fly limb; administer leucine, glutamine, and insulin to the fly; and assess regenerative response in the limb. This protocol was used in Abrams et al., 2021 (doi.org/10.1101/2020.11.21.392720).   

- Fixed deprecation warning for `np.float`. - Fixed spacing of plots in corner plots.

Synthetic signaling circuits could allow engineered cells to sense and control their own population density. The ideal circuit would operate independently of endogenous pathways and be robust to selection pressure for mutations that allow cells to evade population size limits. Here, we show that the plant hormone auxin can be repurposed as an or...

- Fix of a dimensionality bug when using custom kernels.

Hot fix of a bug in covariance matrix generation in Gaussian processes submodule.

- Fixed a bug in periodic kernel - Added linear and polynomial GP kernels.

Minor update: removed references to `line_join` in HoloViews defaults.

- Updated API to match recent versions of CmdStanPy and ArviZ - Streamlined and improved visualizations - Updated bootstrap calculations for new Numpy default RNGs - Stabilized API

- Added datashading to corner plots. - Fixed use of `ray` glyphs to be compatible with Bokeh v. 2.3.0.

Raw data and simulation code for manuscript "A synthetic protein-level neural network in mammalian cells"