Published June 4, 2020 | Version 1.0
Dataset Open

Fluorescence microscopy data of CYK1-GFP embryos in early C. elegans embryo actomyosin flow

  • 1. ROR icon California Institute of Technology
  • 2. ROR icon TU Dresden
  • 3. ROR icon National Center for Genetic Engineering and Biotechnology

Description

Microscopy movies to study the dynamics of cortical CYK-1 foci in C. elegans during early actomyosin flow by imaging fluorescence recovery after photo-bleaching. One-cell C. elegans embryos were obtained from animals grown at room temperature, after 24 hours of feeding with E. coli carrying rga-3 RNAi or L4440 (control) constructs, using a Nikon Spinning Disk Confocal Microscope with an Andor DU-888 camera, 100x oil objective, 50 ms Exposure, ~20 frames per second, and maximum laser intensity.

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Additional details

Created:
September 12, 2022
Modified:
November 28, 2022